Figure standard. Volcano: native 84 × 70 mm, unchanged P<0.05 and |log2FC/effect|≥0.585 thresholds, V19 candidate order within each selection stratum, highlighted points use a 1.5-mm color-core diameter with no outline, 7 pt labels without background boxes, 0.7 pt leader lines. Heatmap: ~120-mm Option-3 wide format, row Z-score, no values inside cells; Top5/Top10 are selected separately in the higher/positive and lower/negative directions, using only candidates meeting P<0.05 and |effect|≥0.585. If a direction has fewer candidates, the actual number is shown without padding. KEGG plots use the correctly matched comparison-specific vendor tables; the source tables contain hsa pathway identifiers and should be rerun with a mouse-specific background before final mechanistic claims. † denotes putative MS1/MS2 library annotation requiring authentic-standard confirmation; uncertain metabolomics features are reported by m/z and RT.